SiR-DNA is a far-red, fluorogenic, cell permeable, low background and highly specific probe for DNA. SiR-DNA is based on the DNA minor groove binder bisbenzimide, it allows the labelling of DNA in live cells with high specificity and low background. SiR-DNA is fluorogenic, cell permeable and highly specific for DNA. Sir-DNA stains the nuclei of live cells without the need for genetic manipulation or overexpression. Its emission in the far red minimizes phototoxicity and sample autofluorescence. This reagent has multiple advantages (far-red, fluorogenic, cell permeable, low background and highly specific probe for DNA) over existing probes such Syto61, DRAQ5 or Vybrant DyeCycle Ruby dyes.
SiR-DNA is compatible with GFP and/or m-cherry fluorescent proteins. It can be imaged with standard Cy5 filtersets. SiR-actin can be used for widefield, confocal, SIM or STED imaging in living cells and tissue. Probe quantity allows 50 – 200 staining experiments.*