Product Uses Include
To study the effects of pharmaceutical compounds on the ratio of Tubulin to Microtubules in cells.
To study the effects of mutated cell lines versus their parent cell line for the change in ratio of Tubulin to Microtubules.
To study the effects of physical alterations of environment on the ratio of Tubulin to Microtubules in cells.
Introduction
The most reproducible and accurate method of determining the amount of microtubule content versus free-tubulin content in a cell population is to use western blot quantitation of microtubule and free-tubulin cellular fractions. The general approach is to homogenize cells in microtubule stabilization buffer, followed by centrifugation to separate the microtubules from free-tubulin pool. Then the fractions are separated by PAGE and tubulin is quantitated by western blot. The final result gives the most accurate method of determining the ratio of tubulin incorporated into the cytoskeleton versus the free-tubulin found in the cytosol. This kit contains all the reagents to perform this assay.