Anti-phosphotyrosine-HRP antibody is an HRP labeled tyrosine phosphorylation specific antibody that is part of the Signal-Seeker™ product line. APY03-HRP phosphotyrosine antibody recognizes a wide range of tyrosine phosphorylated proteins in cells treated with kinase inhibitors, hydrogen peroxide (H2O2) and sodium vanadate (Figure 1) and has been validated for western blot applications. APY03 anti-phosphotyrosine antibody 27B10 has been tested in western blot, immunofluorescence, immunoprecipitation, and ELISA with excellent specificity and low background with strong potent signals.
APY03 is a mouse monoclonal antibody 27B10 that recognizes phosphorylated tyrosine residues. APY03 was raised against a proprietary mixture of phosphotyrosine peptides conjugated to KLH. APY03 can detect less than 10 ng of phosphotyrosine-labeled bovine serum albumin (see Certificate of Analysis [COA]). APY03 is purified by protein G affinity chromatography and is supplied as a lyophilized white powder. Each Lot of antibody is quality controlled to provide a high batch to batch consistency. The Lot specific µg per tube can be found in the Lot specific COA documents. APY03-HRP shows high specificity to phosphotyrosine proteins and does not cross-react with phosphoserine/ threonine proteins a western blot assay (Figures 1).