Material
The GGA3-PBD containing residues 1-316 of GGA3 has been overexpressed in a bacterial expression system. The recombinant protein has a molecular weight of approximately 60kDa and contains a GST-tag at its amino terminus. The purified protein has been conjugated to colored sepharose beads and is supplied as a lyophilized purple powder (Figure 1). One tube of GGA07 should be sufficient for approximately 20-25 assays (see Biologcial Activity Assay on the product datasheet).
The GGA3-PBD protein used for conjugation to the beads was analysed by scanning densitometry of a Coomassie Blue stained 4-20% SDS polyacrylamide gel and determined to be >80% pure (see Figure 2)
The protein is supplied in a glutathione agarose bound format and is shipped lyophilized. The beads are colored for ease of use. This product is used in the Arf1 pull-down activation assay Biochem Kit™ (Cat. # BK032-S) and the Arf6 pull-down activation assay Biochem Kit™ (Cat. # BK033-S).
| Figure 1. The beads provided are colored (a Cytoskeleton innovation in 2004) to easily identify pellet material. |