As part of the Signal-Seeker™ product line, SUMOylation 2/3 affinity beads have been optimized in order to detect endogenous levels of SUMO 2/3 modified proteins, which often represent <1% of the target protein. SUMOylation 2/3 affinity beads comprise an anti-SUMO-2/3 antibody (Clone 11G2) that has been chemically conjugated to Protein G beads. Validation studies done in Hela cells have shown that these beads can immunoprecipitate a wide range of SUMO 2/3 modified proteins without detectable leaching of either heavy or light chains in an IP assay. A comprehensive Signal-Seeker™ SUMOylation 2/3 Detection Kit is also available (BK162) and is recommended for first time users.
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