Rho GAP Protein GST， Rho-GAP蛋白GST
Product Uses Include
In vitro GAP assays with various Rho proteins
Inhibition of Rho proteins in vivo by microinjection
Material
p50RhoGAP (sometimes called Cdc42-GAP or Rho GAP) is a GTPase Activating Protein that catalyzes the hydrolysis of GTP for Rac, Rho, and Cdc42 proteins. It appears to be most active for Cdc42 and least active for Rac.
p50RhoGAP has been purified from a bacterial expression system and is supplied as a GST-fusion protein. This protein is 78 kDa in size and is comprised of the 50 kDa full length p50RhoGAP protein and a 28 kDa GST tag. It is supplied as a lyophilized powder. When reconstituted to 1 mg/ml, the protein is in the following buffer: 2 mM Tris pH 7.6, 0.5 mM MgCl2, 0.5% sucrose and 0.1% dextran. Protein concentration is determined by the Precision Red Advanced Protein Assay Reagent cat. # ADV02.
p50RhoGAP is also available as a purified GST-tagged catalytic (GAP) domain only (Cat. # GAS01)
