FtsZ Protein (E.coli recombinant, 6xHis-tagged) FtsZ蛋白(大肠杆菌重组,6xHis标记)

2020-07-17 14:46:56  浏览:845  作者:管理员

Product uses

  • Identification and characterization of FtsZ binding proteins

  • Characterization of FtsZ dynamics

  • Developing FtsZ ligands that may be used as anti-bacterial agents.

Material
FtsZ is a bacterial cytoskeletal protein that is essential for cell division many prokaryotes (1).  It has been shown to be a bacterial homolog of eukaryotic tubulin, based both on a low sequence identity and a striking structural similarity (2).  Just like eukaryotic tubulin, FtsZ polymerizes as well as binds and hydrolyzes GTP in a polymerization dependent manner.

E. coli FtsZ protein has been purified after over-expression in E.coli. E.c. FtsZ has a 6xHis tag and an approximate molecular weight of 45 kDa (Fig. 1). FTZ05 is provided as a lyophilized protein with buffer and stabilizers.

Purity
Protein purity is determined by scanning densitometry of Coomassie Blue stained protein on a 10% polyacrylamide gel.  FtsZ protein was calculated to be >90% pure (see Figure 1, Lane 2).


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