FtsZ Protein (Staphylococcus aureus recombinant plus 6xHis tag) FTSZ蛋白（金黄色葡萄球菌重组加6xHISTAG）
Product uses
Identification and characterization of FtsZ binding proteins
Characterization of FtsZ dynamics
Developing FtsZ ligands that may be used as anti-bacterial agents.
Material
FtsZ is a bacterial cytoskeletal protein that is essential for cell division many prokaryotes (1). It has been shown to be a bacterial homolog of eukaryotic tubulin, based both on a low sequence identity and a striking structural similarity (2). Just like eukaryotic tubulin, FtsZ polymerizes as well as binds and hydrolyzes GTP in a polymerization dependent manner.
Staphyloccus aureus FtsZ protein has been purified after over-expression in E.coli. S.a. FtsZ has a 6xHis tag and an approximate molecular weight of 55 kDa (Fig. 1). FTZ02 is provided as a lyophilized protein with buffer and stabilizers.
Purity
Protein purity is determined by scanning densitometry of Coomassie Blue stained protein on a 10% polyacrylamide gel. FtsZ protein was calculated to be >90% pure (see Figure 1, Lane 2).
Purity
Purity is determined by scanning densitometry of proteins on SDS-PAGE gels. Samples are >90% pure. No free dye is apparent in the final product.
Figure 1: A 100 µg sample of S.a. FtsZ protein was separated on a 4-20% gradient SDS-PAGE gel and stained with Coomassie blue. Lane 1, 100µg FTZ02. SeeBlue molecular weight markers are from Invitrogen.
