K-Ras4B Mutant Protein: G12D+D38A (human recombinant with 6xHis-tag) K-Ras4B突变蛋白：G12D+D38A（人重组6xHis标签）
 |
The BlastR™ Buffer system allow efficient extraction of proteins from all cellular compartments (Fig. 1). BlastR™ lysis buffer system is compatible with standard colorimetric protein assays unlike Laemmli and 1% denaturing lysis buffer (Fig. 3) It should be noted that BlastR™ Buffer will efficiently break up protein:protein interactions and is therefore not suitable for co-IP applications.
NOTE: Lysates may become viscous due to the release of genomic DNA. The Lysate viscosity can be eliminated after passage over a BlastR™ filter (not included in this kit), providing a user friendly lysate for downstream applications including SDS-PAGE, western blotting, immunoprecipiatations (IPs). The BlastR™ filters are sold separately and can be used with any nuclear lysis buffer system to reduce lysate viscosity by removing genomic DNA from the sample (Fig. 2)
|
Product Uses Include
Remove genomic DNA
Suitable for SDS-PAGE Application
Suitable for IP Applications
Sample Kit Components (50 lyses)
BlastR™ Lysis buffer
BlastR™ Dilution buffer
