H-Ras Protein; wild type 6xHis Tag H-Ras蛋白；野生型6xHis标签
Product Uses Include
Ras biochemistry
Ras GTPase assays
Ras nucleotide exchange assays
Ras binding studies
Material
The human H-Ras protein has been produced in a bacterial expression system. The protein is supplied as a lyophilized powder. When it is reconstituted in distilled water to 1 mg/ml, the protein is in the following buffer: 2 mM Tris pH 7.6, 0.5 mM MgCl2, 0.5% sucrose, 0.1% dextran. Protein concentration is determined by the Precision Red Advanced Protein Assay Reagent, Cat. # ADV02.
The recombinant protein is approximately 28 kDa, consisting of the H-Ras protein plus a histidine tag in the amino-terminus.
For other forms of Ras as well as many other purified small G-proteins, see our main small G-protein product page.
Purity
Purity is determined by scanning densitometry of proteins on SDS-PAGE gels. His-H-Ras samples are >80% pure.
