RhoC His Protein: wild type RhoC-His蛋白:野生型

2020-07-17 17:07:40  浏览:877  作者:管理员


Product Uses Include

  • RhoC biochemistry

  • RhoC GTPase assays

  • RhoC nucleotide exchange assays

  • RhoC binding studies

Material
The human RhoC protein has been produced in a bacterial expression system. The protein is supplied as a lyophilized powder. When it is reconstituted in distilled water to 1 mg/ml, the protein is in the following buffer: 2 mM Tris pH 7.6, 0.5 mM MgCl2, 0.5% sucrose, 0.1% dextran. Protein concentration is determined by the Precision Red Advanced Protein Assay Reagent, Cat. # ADV02.

The recombinant protein is approximately 30 kDa, consisting of the RhoC protein plus a histidine tag in the amino-terminus.

For other forms of Rho proteins as well as many other purified small G-proteins, see our main small G-protein product page.

Purity
Purity is determined by scanning densitometry of proteins on SDS-PAGE gels. His-RhoC samples are ~90% pure.


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