Microtubules (Taxol Stabilized and Lyophilized) 微管（紫杉醇稳定和冻干）
Product uses
Substrate for activation of kinesin ATPases (see Fig. 1)
Determination of IC50s for kinesin inhibitors
Used in Cytoskeleton, Inc’s kinesin ELIPA and End point ATPase assays (Cat. # BK060 & BK053)
Substrate for discovery and characterization of microtubule binding proteins (MAPs) (see Fig. 2)
Ideal for HTS applications (inquire for bulk rates)
Material
Stabilized microtubules (MTs) are supplied as a lyophilized powder. Microtubules have been prepared from porcine brain tubulin protein that is greater than 99% pure (Cat. # T240). These stringently quality controlled MTs provide highly reliable and reproducible results in assays that require MT substrates. The average MT length in this product is 2 µm. Using these pre-formed microtubules in assays depending on microtubules gives very reproducible results. In a microtubule activated kinesin ATPase assay (see Fig. 1), the CV between samples (Vmax) was less than 5% and the CV of Vmax between assays using different batches of MT001 was less than 8%.
