MICAL (MIC01) is an intracellular flavoprotein monoxygenase, conserved from insects to mammals, that functions as a catalyst for oxidation-reduction (redox) reactions. Terman’s group showed that MICAL interacts with F-actin and uses NADPH as a cofactor to oxidize actin at Met44 and Met47. As part of the MOXtrue™ product line, Human MICAL1 protein (MIC01) [accession # Q8TDZ2 (MICA1_HUMAN)] has been produced and purified from a bacterial expression system. The recombinant protein is N-terminally 6x Histidine tagged and is comprised of the Redox and CH domains (aa: 1-612) of the full length protein. Validation studies have shown that MIC01 can oxidize actin which will reduce subtilisin A cleavage by >90% in limited proteolysis assays. MIC01 has also been validated for the production of oxidized actin which can be used in downstream applications such as sedimentation assays and actin polymerization assays.
To learn more about using MICAL as a research tool see our Datasheet
To learn more about the MICAL/MsrB/Actin physiological redox system see our Newsletter
Each lot of purified protein is quality controlled to provide high batch to batch consistency, see COA documents.