Product Uses Include
Cell invasion assays (1)
FACS analysis of laminin binding cells (2)
Material
Laminin-1 is purified from EHS tumor tissue and is free of the laminin binding protein entactin which is a common contaminant in some laminin preparations (150 kDa). Protein purity is determined by scanning densitometry of Coomassie Blue stained protein on a 4-20% polyacrylamide gel. The laminin is >90% pure (Figure 1).
The protein is modified to contain covalently linked HiLyte 488TM dyes (3) at random surface lysines. An activated ester of HiLyte 488TM is used to label the protein. Labeling stoichiometry is determined by spectroscopic measurement of protein and dye concentrations. Final labeling stoichiometry is 2-5 dyes per protein molecule (Figure 2). The material is guaranteed to contain <15% of free dye and >85% of dye conjugated to laminin. HiLyte 488TM laminin can be detected using a filter set of 502nm excitation and 527nm emission.
Laminin runs as individual subunits on SDS-PAGE with an apparent molecular weight of 400 and 225 kDa (Figure 1). LMN02 is supplied as an off white lyophilized powder. Each vial of LMN02 contains 20 µg protein.