Product uses
Identification and characterization of FtsZ binding proteins
Characterization of FtsZ dynamics
Developing FtsZ ligands that may be used as anti-bacterial agents.
Material
FtsZ is a bacterial cytoskeletal protein that is essential for cell division many prokaryotes (1). It has been shown to be a bacterial homolog of eukaryotic tubulin, based both on a low sequence identity and a striking structural similarity (2). Just like eukaryotic tubulin, FtsZ polymerizes as well as binds and hydrolyzes GTP in a polymerization dependent manner.
Streptococcus pneumoniae FtsZ protein has been purified after over-expression in E.coli. S.p. FtsZ has a 6xHis tag and an approximate molecular weight of 45 kDa (Fig. 1). FTZ03 is provided as a lyophilized protein with buffer and stabilizers.
Purity
Protein purity is determined by scanning densitometry of Coomassie Blue stained protein on a 10% polyacrylamide gel. FtsZ protein was calculated to be >90% pure (see Figure 1, Lane 2).
Purity
Purity is determined by scanning densitometry of proteins on SDS-PAGE gels. Samples are >90% pure. No free dye is apparent in the final product.