Product Uses Include
Observation of fibronectin matrix assembly (1)
Cell invasion assays
Bioengineering; study of cell adhesion to polymers (2)
Material
Fibronectin is purified from bovine plasma. Protein purity is determined by scanning densitometry of coomassie blue stained protein on a 4-20% polyacrylamide gel. Biotinylated fibronectin is >80% pure (Figure 1).
The protein is modified to contain covalently linked biotins at random surface lysines. A long-chain activated ester of biotin [biotin-XX, succinimidyl ester] is used to label the protein. Labeling efficiency is determined by the ability to detect 10 ng biotinylated fibronectin using alkaline phosphatase conjugated streptavidin (Figure 2).
Fibronectin runs as individual subunits on SDS-PAGE with an apparent molecular weight of 230 kDa. FNR03 is supplied as a white lyophilized powder. Each vial contains 20 µg protein.