Product Uses
Measurement of calcium activated myosin ATPase activity when bound to thin filaments.
Identification/characterization of proteins or small molecules that affect the TT complex regulation and myosin ATPaseactivity
Identification/characterization of proteins or small molecules that affect myosin / F-actin interaction
Material
The Tropomyosin / Troponin protein (TT) complex has been purified from bovine cardiac muscle (1). The TT complex is composed of five proteins Tropomyosin alpha: Tropomyosin beta: TroponinC : Troponin I : Troponin T in a stoichiometric ratio of 1:1:1:1:1, see Figure 1. The complex has been determined to be biologically active in an F-actin / calcium activated myosin ATPase assay (see biological activity assay). The complex is supplied as a white lyophilized powder.
Figure 1—Schematic diagram of muscle thin filament.
