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Product Uses
IC50 & EC50 determinations for anti-tubulin ligands.
Characterization of tubulin binding proteins.
Materials
Tubulin protein has been purified from Caki-1 tumor tissue by an adaptation of the method of Davis et al. (2), using anion exchange chromatography followed by a cycle of polymerization/depolymerization. TM001 is supplied in 250 μg aliquots in a lyophilized format in 10 mM Na-PIPES buffer pH 6.9, 0.25 mMMgCl2, 0.1 mM GTP, 5% sucrose and 1% Ficoll-400K. Tubulin consists of a heterodimer of one alpha and one beta isotype, eachtubulin isotype is 55 kDa in size, SDS-PAGE analysis shows tubulin running as a 55 kDa species (see Figure 2). Typically, the molar equivalent of tubulin is defined as the heterodimer which has a molecular weight of 110 kDa.
