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The BlastR™ Buffer system allow efficient extraction of proteins from all cellular compartments (Fig. 1). BlastR™ lysis buffer system is compatible with standard colorimetric protein assays unlike Laemmli and 1% denaturing lysis buffer (Fig. 3) It should be noted that BlastR™ Buffer will efficiently break up protein:protein interactions and is therefore not suitable for co-IP applications.
NOTE: Lysates may become viscous due to the release of genomic DNA. The Lysate viscosity can be eliminated after passage over a BlastR™ filter (not included in this kit), providing a user friendly lysate for downstream applications including SDS-PAGE, western blotting, immunoprecipiatations (IPs). The BlastR™ filters are sold separately and can be used with any nuclear lysis buffer system to reduce lysate viscosity by removing genomic DNA from the sample (Fig. 2)
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Product Uses Include
Remove genomic DNA
Suitable for SDS-PAGE Application
Suitable for IP Applications
Sample Kit Components (50 lyses)
BlastR™ Lysis buffer
BlastR™ Dilution buffer