Product Uses
Study inhibitors of SOS2 GTP exchange activity
Biochemical characterization of SOS2 specific protein interactions
Study of SOS2 GEF activity with different GTPases
Compare differences in SOS1 and SOS2 exchange activities
Materials
The human SOS2 exchange domain (563-1051) protein (SOS2-ExD) has been produced in a bacterial expression system. The recombinant protein contains a 6x histidine tag (His-tag) at its amino terminus. The approximate molecular weight of the SOS2-ExD protein is 61 kDa. The protein is supplied as a lyophilized white powder.
Storage and Reconstitution
The protein should be reconstituted to 50 mM (3.03 mg/ml) by the addition of 33 µl of nanopure water. The protein will be in the following buffer; 17 mM Tris pH 7.5, 17 mM NaCl, 0.2 mM MgCl2, 2.3% sucrose, 0.3% dextran. In order to maintain high biological activity of the protein, it is strongly recommended that the protein solution should be aliquoted into "experiment sized" aliquots and snap frozen in liquid nitrogen. The protein can be stored at -70°C for 6 months. Avoid repeated freeze-thaw cycles. The lyophilized protein is stable at 4°C for 1 year when stored desiccated (<10% humidity).