Product Uses Include
In vivo actin polymerization studies (micro-injection into non-muscle cells)
In vitro motility studies using fluorescent F-actin and non-muscle myosins
Material
Non-muscle actin purified from human platelets (Cat. # APHL99) has been modified to contain covalently linked rhodamine at random surface lysine residues. An activated ester of rhodamine is used to label the protein. The labeling stoichiometry has been determined to be 0.4 dyes per actin monomer. Rhodamine non-muscle actin has an approximate molecular weight of 43 kDa. Rhodamine non-muscle actin is supplied as a pink lyophilized powder. The lyophilized protein is stable for 6 months wh en stored desiccated to <10% humidity at 4°C. The protein should be reconstituted to 10 mg/ml with distilled water, it will then be in the following buffer: 5 mM Tris-HCl pH 8.0, 0.2 mM CaCl2, 0.2 mM ATP, 5% sucrose, and 1% dextran.