Product Uses Include
IC50 & EC50 determinations for anti-tubulin ligands.
Microtubule binding studies
Tubulin monomer binding studies
HDAC6 studies
Microtubule activated kinesin ATPase assays
Material
Tubulin protein has been purified from porcine brain by an adaptation of the method of Shelanski et al. (1), Further purification to >99% purity was achieved by cation exchange chromatography. Tubulin is supplied at 10 mg/ml as a frozen liquid in G-PEM (General tubulin buffer (Cat. # BST01) with 1 mM GTP (Cat. # BST06)).
This product is also available in a lyophilized, fully active format (Cat. # T240). The lyophilized format tubulin has many advantages over the frozen format. T240 costs less and since it allows for ambient temperature shipping, shipping is also significantly less costly. In addition, the lyophilized format allows for simpler (+4°C desiccated vs -70°C) and longer storage.
T238P is simular to T237 when 10% glycerol is added. >99% pure tubulin is also available in a convenient lyophilized pre-formed microtubule format (Cat. # MT001) for use in e.g. kinesin ATPase assays and microtubule binding studies.
Purity
Purity is determined by scanning densitometry of proteins on SDS-PAGE gels. Samples are >99% pure.