Product Uses Include
RhoA effector and GAP identification and binding studies
Activation of RhoA effectors in vitro and in vivo
Stimulation of RhoA phenotype by microinjection into cells
Material
The constitutively active form of human RhoA protein contains a glutamine to leucine substitution at residue 63. The common name for this mutant is RhoA(Q63L) or L63RhoA. The leucine substitution prevents endogenous and GAP-stimulated GTPase activity of RhoA, hence the protein is always in the active, GTP-bound, state. The corresponding mutation in Ras (Ras(Q61L)) behaves as an oncogenic mutation.