Product Uses Include
Precipitation of active Rac and Cdc42 from cell lysates
Inhibition of Rac and Cdc42 activity in vitro
Blocking of Rac and Cdc42 function in vivo by microinjection
Material
The Rac/Cdc42 (p21) binding domain (PBD) of the human p21 activated kinase 1 protein (PAK) protein has been expressed as a GST-fusion protein in E. coli. This protein binds binds specifically to GTP-bound, and not GDP-bound, Rac and Cdc42 proteins. The domain can therefore be used to specifically precipitate active, GTP-bound Rac and Cdc42 as well as to specifically block the activity of Rac and Cdc42 in vitro and in vivo.
The GST-PAK-PBD contains residues 67-150 of PAK. This region includes the highly conserved CRIB motif plus sequences required for the high affinity interaction with GTP-Rac and GTP-Cdc42.
The protein is supplied as 250 µg of lyophilized product and the protein is also available in a bead bound format (Cat. # BK034 and <a href="https://www.cytoskeleton.com/pak01" {{store"="" url="BK035" style="box-sizing: border-box; text-decoration-line: none; color: rgb(0, 136, 204) !important;">BK035, repectively)
Purity
Protein purity is determined by scanning densitometry of Coomassie Blue stained protein on a 12% SDS polyacrylamide gel. GST-PAK-PBD protein is >80% pure (see Figure 1).
