Product uses
Substrate for activation of kinesin ATPases (see Fig. 1)
Determination of IC50s for kinesin inhibitors
Used in Cytoskeleton, Inc’s kinesin ELIPA and End point ATPase assays (Cat. # BK060 & BK053)
Substrate for discovery and characterization of microtubule binding proteins (MAPs) (see Fig. 2)
Ideal for HTS applications (inquire for bulk rates)
Material
Stabilized microtubules (MTs) are supplied as a lyophilized powder. Microtubules have been prepared from porcine brain tubulin protein that is greater than 99% pure (Cat. # T240). These stringently quality controlled MTs provide highly reliable and reproducible results in assays that require MT substrates. The average MT length in this product is 2 µm. Using these pre-formed microtubules in assays depending on microtubules gives very reproducible results. In a microtubule activated kinesin ATPase assay (see Fig. 1), the CV between samples (Vmax) was less than 5% and the CV of Vmax between assays using different batches of MT001 was less than 8%.